| Id: | 3287
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| Autor: | Soares, Marcelo A; Teixeira, Paulo R. |
| Título: | A specific subtype C of human immunodeficiency virus type 1 circulates in Brazil.
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| Fonte: | AIDS;17(1):11-21, jan.2003. tab.
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| Resumo: | OBJECTIVE: To characterize the subtype C strains of HIV type 1 that circulate in Brazil, especially those originated from the southern part of the country. DESIGN AND METHODS: One hundred and twelve HIV-1-positive subjects had their plasma viral RNA extracted. Protease (PR) and reverse transcriptase (RT) genomic regions were polymerase chain reaction-amplified and sequenced for subtype determination. Subtype C strains were selected and compared to other strains of this subtype from the database, and specific amino acid signature patterns were searched. RESULTS: Brazilian subtype C viruses form a very strong monophyletic group when compared to subtype C viruses from other countries and presented specific signature amino acids. Recombinants between subtype C and B viruses have been documented in areas of co-circulation. The incidence of primary PR and RT inhibitor resistance mutations in drug-naïve subjects was observed. An increasing number of secondary resistance mutations was also seen, some of which are characteristic of subtype C-related sequences. CONCLUSIONS: Introduction of subtype C of HIV-1 in Brazil was likely a single event of one or a mixture of similarly related strains. Recombination between subtype C and B viruses is an ongoing process in the country. Primary and secondary drug resistance mutations were observed, although some of the secondary mutations could be associated with subtype C molecular signatures. Subtype-specific polymorphisms of PR and RT sequences found in this subtype C Brazilian variant might influence this emergence and have an impact on HIV treatment and on vaccine development in the country.
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| Descritores: | Sequência de Aminoácidos
Resistência Viral a Drogas/genet
Infecções por HIV/epidemiol
Infecções por HIV/virol
Protease de HIV/genet
HIV-1/clas
HIV-1/genet
HIV-1/isol
Transcriptase Reversa do HIV-1/genet
- Brasil/epidemiol
África
India
Mutação
Reação em Cadeia da Polimerase/métodos
Variação (Genética)
Dados de Sequência Molecular
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| Limites: | Humano
Masculino
Feminino
Adolescente
Adulto
Meia-Idade
Support, Non-U.S. Gov't
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| Localização: | BR1310.1; PAPROF |
